Dr. Melody "pepsi" holmquist
Career summary
Currently teaching quantitative instrumental analysis and fundamental chemistry courses at National Technical Institute for the Deaf (NTID), a college at Rochester Institute of Technology (RIT) since August 2020. Earned a Ph.D. in biochemistry from the Ohio State University Program with 5+ years of experience in mass spectrometry and 2+ years of university teaching experience. For research, I specialize in protein and RNA native mass spectrometry, determining the interactions and stoichiometric of ligand-protein complexes and nucleic acid-protein complexes, and elucidating allosteric mechanisms using custom microscopic thermodynamic binding models. Looking forward to apply my expertise to biological complexes using mass spectrometry.
Relevant Experience
2021 – Present
Visiting Assistant Professor, Dept. of Science and Mathematics , National Technical Institute for the Deaf/Rochester Institute of Technology – Rochester, NY
Visiting Assistant Professor, Dept. of Science and Mathematics , National Technical Institute for the Deaf/Rochester Institute of Technology – Rochester, NY
Education
2020
PhD, Advisor: Dr. Mark Foster and co-advisor, Dr. Vicki Wysocki,
The Ohio State University, Columbus, OH
Ohio State Biochemistry Program (OSBP)
2012
BS, Advisor: Dr. Lea Michel,
Rochester Institute of Technology, Rochester, NY
Department of Biochemistry
PhD, Advisor: Dr. Mark Foster and co-advisor, Dr. Vicki Wysocki,
The Ohio State University, Columbus, OH
Ohio State Biochemistry Program (OSBP)
2012
BS, Advisor: Dr. Lea Michel,
Rochester Institute of Technology, Rochester, NY
Department of Biochemistry
publications
Pichichero, M.E., Labbe, N., Kasper, A., Pilo, I., Jackson, N., Jones, S.P., Perdue, J., Pryharski, K., Holmquist, M.L., Gleghorn, M.L., Kaur, R., Michel, L.V. (2022) Haemophilus influenzae Protein D antibody suppression in a multi-component vaccine formulation, FEBS Open Bio, doi: 10.1002/2211-5463.13498
Jones, S.P., Cook, K.H., Holmquist, M.L., Almekinder, L.J., Delaney, A.M., Charles, R., Labbe, N., Perdue, J., Jackson, N., Pichichero, M.E., Kaur, R., Michel, L.V., Gleghorn, M.L. (2022) Vaccine target and carrier molecule nontypeable Haemophilus influenzae protein D dimerizes like the close Escherichia coli GlpQ homolog but unlike other known homolog dimers, Proteins: Structure, Function, and Bioinformatics, doi: 10.1002/prot.26418
Holmquist, M., Ihms, E.C., Gollnick, P., Wysocki, V.H., and Foster, M.P. (2020) Population distributions from native mass spectrometry titrations reveal nearest-neighbor cooperativity in the ring-shaped oligomeric protein TRAP, Biochemistry, doi: 10.1021/acs.biochem.0c00352
Sgheiza, V., Novick, B., Stanton, S., Pierce, J., Kalmeta, B., Holmquist, M.F., Grimaldi, K., Bren, K.L., and Michel, L.V. (2017) Covalent bonding of heme to protein prevents heme capture by nontypeable Haemophilus influenzae, FEBS Open Bio, 7, 1778-1783, doi: 10.1002/2211-5463.12324.
Jones, S.P., Cook, K.H., Holmquist, M.L., Almekinder, L.J., Delaney, A.M., Charles, R., Labbe, N., Perdue, J., Jackson, N., Pichichero, M.E., Kaur, R., Michel, L.V., Gleghorn, M.L. (2022) Vaccine target and carrier molecule nontypeable Haemophilus influenzae protein D dimerizes like the close Escherichia coli GlpQ homolog but unlike other known homolog dimers, Proteins: Structure, Function, and Bioinformatics, doi: 10.1002/prot.26418
Holmquist, M., Ihms, E.C., Gollnick, P., Wysocki, V.H., and Foster, M.P. (2020) Population distributions from native mass spectrometry titrations reveal nearest-neighbor cooperativity in the ring-shaped oligomeric protein TRAP, Biochemistry, doi: 10.1021/acs.biochem.0c00352
Sgheiza, V., Novick, B., Stanton, S., Pierce, J., Kalmeta, B., Holmquist, M.F., Grimaldi, K., Bren, K.L., and Michel, L.V. (2017) Covalent bonding of heme to protein prevents heme capture by nontypeable Haemophilus influenzae, FEBS Open Bio, 7, 1778-1783, doi: 10.1002/2211-5463.12324.
upcoming
Holmquist, M., Jones, B.J., Gollnick, P., Wysocki, V.H., and Foster, M.P. (2024) Native MS informs us of mechanistic details of heterotropic allostery of RNA binding to Trp Attenuation RNA-binding Protein.
Holmquist, M., Harvey, S., Wysocki, V.H., and Foster, M.P. (2024) Surface-induced dissociation mass spectrometry reveals the disassembly pathway of Anti-TRAP dodecamers.
Holmquist, M., Harvey, S., Wysocki, V.H., and Foster, M.P. (2024) Surface-induced dissociation mass spectrometry reveals the disassembly pathway of Anti-TRAP dodecamers.
Professional Membership
ASMS American Society for Mass Spectrometry, 2016 - present
ACS American Chemistry Society, 2019 - present
ASMS American Society for Mass Spectrometry, 2016 - present
ACS American Chemistry Society, 2019 - present
